Bacteria, which has high growth rate as compared to fungi has good potential to be used in cellulase production. Cellulases are inducible enzymes that are synthesized by a large number of microorganisms during their growth on cellulosic materials. Optimization of the fermentation medium for maximum cellulase production was carried out. The effect of various fermentation conditions for cellulase production through shakeflask culture was. The solid cellulosic waste can be treated efficiently. Pdf optimization of cellulase production from bacteria isolated. The sesame seed residues were collected in sterile plastic bags and numbered. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose cmc agar plate were identified as cellulase producing bacteria. The prospects of cellulaseproducing bacteria for the. These cellulaseproducing bacteria can be applied for use in a wide variety of industries such as dessert production. The isolated by2 strain rapidly grew and produced cellulase. Isolation, production, purification and characterization.
In the present investigation, we aim to isolate the two novel cellulase producing fungi aspergillus niger and trichoderma sp. Cellulase production from various waste cellulosic materials using different cellulolytic microfungi is being vigorously studied for cost reduction strategies. Cellulase enzymes are isolated from fungi present in higher termite soil. Bioethanol production with cellulase enzyme from bacillus. Development of a lowcost cellulase production process. Afterthat, the cellulase enzyme was applied for purification and characterization of different parameters and purify enzyme was applied for the ethanol production and industrial exploitation which discuss. Optimization of fermentation condition for cellulase. Pdf identification of cellulaseproducing bacteria from soil in. Pdf cellulase production by bacillus subtilis isolated. Optimization of culture conditions for production of cellulase by stenotrophomonas maltophilia. Immobilized coldactive cellulase can effectively hydrolyze the cellulose of kelp residue, which is a valuable component of cellulose bioethanol production and will have broad implications in the development of the ethanol industry in china. Inducible cellulase production from an organic solvent. Fungal cellulase is an abundant resources,is a potential raw material for the microbial production of food, fuel etc. Cloning and recombinant expression of a cellulase from the cellulolytic strain streptomyces sp.
Bacteria are important sources of cellulases with various industrial and biotechnological applications. P, india 2institute of transgene life sciences, lucknow, u. Cellulase can be produced by fungi, bacteria or actinomycetes, but the most common producer is fungi. Cellulose is mainly degraded by cellulase enzyme which is commonly produced by bacteria and fungi 6.
Microbial cellulases production, applications and challenges. Annadurai research and development centre, bharathiyaruniversity, coimbatore641 041. Pdf microbial cellulasesproduction, applications and. Screening, purification and characterization of cellulase. Rhizobium trifolii cellulase was induced by a variety of polysaccharides, including celluloses and hemicelluloses. The effects of water content, dosage of wheat bran and initial ph value in solid substrate on cellulase synthesis were studied in shallow tray fermentors. For commercial production of these enzymes, the focus is on utilization of agroresidual wastes along with development of efficient bioprocess strategies to obtain highenzyme titers. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production. Screening and identification of cellulase producing bacteria isolated from oil palm meal saowapar khianngam1, yupa pootaengon1, taweesak techakriengkrai2, somboon tanasupawat3 1faculty of animal sciences and agricultural technology, silpakorn university, phetchaburi it campus, phetchaburi 76120, thailand. Isolation and identification of a cellulolytic bacterium from the. Cellulase production from species of fungi and bacteria. Improving the economics of such processes will involve cost reduction in cellulase production which may be. Cloning and recombinant expression of a cellulase from the. In contrast, a bacterial culture is simple, grows rapidly, and has a short generation time, and other.
Production of cellulase enzyme from isolated fungus and. Bacterial cellulase production have been mostly recorded under submerged fermentation conditions, whereas solidstate fermentation for. The isolated fungal cultures were used to know their potential for cellulase production and activities. Screening for cellulase production can be done by enrichment growth on microcrystalline cellulose as a sole source of carbon, followed by the extraction of 16s rdnarna to determine the molecular community structure of the environment and. This study highlights fungal and bacterial strains for the production of cellulases and its industrial applications. Isolation and screening of cellulolytic bacteria from soil. Among bacteria, pseudomonas fluorescens is the best cellulase producer among the four followed by bacillus subtilis, e. Cellulaseproducing bacteria were isolated from soil and identified as. In industry, these enzymes have found novel applications in the. The production of cellulase has been reported from a wide variety of bacteria 22 and fungi 4, 34. This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste molasses and characterization by morphological and biochemical analysis.
Microbial cellulases production, applications and challenges rajeev k sukumaran, reeta rani singhania and ashok pandey biotechnology division, regional research laboratory, industrial estate p o, trivandrum 695 019 microbial cellulases find applications in various industries and constitute a major group of the industrial enzymes. The prospects of cellulaseproducing bacteria for the bioconversion of lignocellulosic biomass. The production of cellulase byrhizobium species was studied. Cellulase is a multienzyme,system having 3 types of. When compared to the anaerobic bacteria, aerobic bacteria can perform better cellulase production of around 90%95% of bacterial cellulose degradation. Cellulase production by solid state fermentation on. Cellulasestypes and action five general types of cellulases based on the type of reaction catalyzed. During the past few years, the first industrialscale cellulosic ethanol plants have been inaugurated. Bacillus subtilis au1 was found to produce carboxymethylcellulase cmcase and avicelase activities in the culture supernatant when grown on a variety of carbohydrates as major carbon source. The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. Optimization and molecular identification of novel cellulose degrading bacteria isolated from egyptian environment.
Maximum cmcase production was obtained in a liquid medium containing 0. Isolation, screening, and identification of the cellulaseproducing bacteria. Pdf bioconversion of cellulose, mainly by bacteria and fungi, plays a key role in the global carbon cycle. Download as pptx, pdf, txt or read online from scribd. The results were compared with bergeys manual of determinative bacteria 19.
The residues were serially diluted using sterile distilled water and cultured on basal medium agar plates. Cellulase production by bacillus subtilis isolated from cow dung. In energy deficient world, cellulases play a major role for the production of alternative energy resources utilizing lignocellulosic waste materials for bioethanol and biogas production. Cellulaseproducing bacteria were isolated from soil and identified as pseudomonas fluorescens, bacillus subtilis, e. Cellulase activity enhancement of bacteria isolated from. The utilization of cheaper substrates reduces cost of cellulase production and there is the need for cellulolytic microbes capable of degrading lignocellulosic materials. Cold adaptation in bacteria with special focus on cellulase production and its potential application.
Optimization of cellulase production from bacteria isolated from soil. Screening for cellulase production can be done by enrichment growth on microcrystalline cellulose as a sole source of carbon, followed by the extraction of 16s rdnarna to determine the molecular community structure of the environment and analyze. Introduction cellulose are produced mainly in plants. However, filamentous fungi are preferred for commercial enzyme production, because the level of the enzymes produced by these cultures is higher than those obtained from yeast and bacteria 8.
Isolation and characterization of novel cellulase and pectinase producing bacteria. Their sources and applications chapter pdf available july 2017 with 1,6 reads. Further, purification of cellulase was carried by ammonium sulfate. The production of biobased products and bioenergy from less costly renewable lignocellulosic materials would bring benefits to the local economy, environment, and national energy security zhang, 2008. Optimization for the production of cellulase enzyme from. Isolation and characterization of novel cellulase and. Kaliwal department of studies in biotechnology and microbiology, karnatak university, dharwad 580 003, india corresponding author abstract introduction enzymes are delicate protein molecules necessary for life. Although a large number of microorganisms fungi, bacteria and actinomycetes are capable of degrading. Therefore, this study was aimed to isolate and identify bacteria from rumen fluid, and evaluate wheat straw, sorghum straw and bagasse for cellulase production. High cost of cellulase is mainly due to the substrates used in production, and also the slow growth rate of fungi. Optimization of culture conditions for production of.
Production and purification of the cellulase enzymatic. Isolation and characterization of cellulaseproducing. Depending on the region, transport of cellulases from a central production facility to a biorefinery may. Rumen fluid were collected from cattle, goat and sheep. Cellulase enzyme has been used for the bioconversion of lignocellulosic to these useful products. Screening isolation and characterization of cellulase. It describes the current knowledge of cellulase production by submerged fermentation and solid state fermentation, properties of cellulase and cloning and expression of cellulase gene. Optimization of cellulase production from bacteria. Acca2 and its application in biomass saccharification. Subject cellulases and pectinases industrial applications microbial cellulases and pectinases.
Isolation and characterization of cellulase producing. Isolation of cellulose degrading bacteria from rumen and. Optimization of cellulase production by enhydrobacter sp. Cellulase is composed of more than one distinctive enzymes which can degrade cellulose and is produced by a wide variety of fungal and bacterial species and the enzyme is transported across the cell membrane to the outside environment. Pdf microbial cellulases find applications in various industries and constitute a. The current study is concerned with the production and partial characterization of cellulase enzyme from bacillus sp. Jain central pulp and paper research institute, saharanpur, u. Isolation and characterization of cellulase producing bacteria from soil basavaraj i. They are produced by fungi, bacteria, protozoans, plants, and animals. Download fulltext pdf download fulltext pdf microbial cellulase and xylanase.
Screening isolation and characterization of cellulase producing microorganisms from soil. Optimization of cellulase production by soil bacteria using statistical design maninder dhaliwal, s. The name is also used for any naturally occurring mixture or complex of various such enzymes, that act serially or synergistically to decompose cellulosic material. The culture conditions like ph, temperature, carbon sources, and nitrogen sources were optimized. Cellulase is an important enzyme in presentday of industrial biotechnology. The analysis of nutrient consumption, production of the fungus, the biosynthesis of enzyme complex of cellulase and other relevant data of the fermentation will be investigated in order to establish a strategy for protein synthesis, and starting enzymatic cellulase complex purification at the fermentation level. Optimization and molecular identification of novel.
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. Production of cellulase enzyme from isolated fungus and its application as efficient refining aid for production of security paper chetna gupta, priyanka jain, dhermander kumar, a. Cellulase production by these strains was optimized by controlling different environmental and nutritional factors such as. Isolation and screening of thermophilic cellulaseproducing bacteria. The present study was to isolate a potential organicsolventthermostable alkalophilic cellulase producing bacteria from natural ecosystem. More department of microbiology, birla college of arts, commerce and science, birla college road, kalyan w, thane43204, m. Screening, purification and characterization of cellulase from. Screening methods for cellulase producing microorganisms. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Production of cellulase enzymes by bacteria and the factors affecting the process. Screening and identification of cellulase producing. Endocellulases breaks internal bonds to disrupt the crystalline structure of cellulose and expose individual cellulose polysaccharide chains exocellulase cleaves 24 units from the ends of the exposed chains produced by endocellulase, resulting in the. This study focuses on the isolation and screening of cellulaseproducing bacteria from sugar industry waste molasses and characterization by morphological and biochemical analysis.
Cellulase research has been concentrated mostly in fungi but there is increasing interest in cellulase production by bacteria due to their higher growth rate and thermo stable and alkali stable. Premalatha n, gopal no, jose pa, anandham r and kwon sw 2015 optimization of cellulase production by enhydrobacter sp. Cellobiose and myoinositol also allowed enzyme expression but mannitol prevented it at concentrations higher than 0. Production of cellulase by aspergillus niger under.
44 1248 943 115 1153 227 1357 1145 874 1011 496 1470 513 892 1357 273 1559 219 1342 118 1288 266 1216 645 1387 793 439 1123 916 984 763 537 940 538 976 978 568 428 779 1348 118 138